The discussion was moderated by Belen Fouz and Alain LeBreton

Generally, laboratory capacities for diagnostics of Vibrio infections are overall good.

For different Vibrio infection, clinical examination is similar, however, in the case of V. harveyi, neurological signs and mucous in the intestine is specific. Temperatures above 20^oC clinical signs are evident.

Isolation on Marine agar and TSA supplemented with NaCl is same for all vibriosis. For V. harveyi additional samples should be taken from the brain.

Identification using phenotypic methods may lead to misidentification with V. alginolyticus.

Identification using MALDI TOF is not strong due to low database for this V. harveyi.

Specific PCR method targeting the ToxR gene is a highly specific method.

Vaccination trials are in progress with different isolates and it seems that i/p application is necessary.

Diagnostic of Tenacibaculum spp.

The suspicion is based on the clinical signs and stained smears of the lesions.

Diagnostics should be done by a sampling of the lesions and seeding of material to FMM or MA for cultivating bacterial colonies. The identification to the genus is possible using API while species are identified by MALDI TOF or 16S rRNA sequencing.

Some specific primers for Tenacibaculum maritimum exist with different specificity

Antimicrobial sensitivity testing is carried out on FMM agar prepared with seawater and the results could be obtained in 2 days.

Swabs should be taken into a transport medium (with gentamycin) and the identification should be performed in the laboratory