\u2022 Large individual variation in total muscle fat content (CV=0.31) were found. Similarly, large individual variation was found in total liver fat content (0.13). Gilthead seabream<\/strong><\/p>\n \u2022 Large individual variation in total muscle fat content (CV=0.20) were found. Genotyping<\/strong><\/p>\n The combined-species 60K SNP array for the European seabass and the gilthead seabream (the MedFish SNP chip), which was developed in a collaboration between the MedAID and PERFORMFISH consortia, showed that 90% of the SNPs in European seabass and 88% of the gilthead seabream were classified as high quality. These figures are good and normal for the first version of a SNP chip.<\/p>\n The MedFish chip has been used to genotype ~10000 European seabass and gilthead seabream within and outside the MedAID and PERFORMFISH consortia. This shows the relevance of the chip to the European RTD and breeding industry sectors of European seabass and gilthead seabream.<\/p>\n In MedAID, the SNP chip will be used for genomic analysis (gene mapping and genomic selection) of production traits, in particular lipid-related traits, for population genetic studies and for estimation of genotype x environment interactions in seabream.<\/p>\n
\n\u2022 Promising individual differences were observed for fatty acid content in the muscle (CV= 0.04-0.15) and in the liver (0.56 \u2013 1.10) on fish fed the same diet.
\n\u2022 Health-associated fatty acids EPA and DHA were in the top ten most abundant fatty acids in the muscle and the liver.
\n\u2022 Correlations between absolute content of fatty acids in the muscle deviated largely from the correlations of absolute fatty acid content in liver. This is due to differences in metabolic capacity between these tissues.
\n\u2022 The scatter plots of the relative measures of the contents of the fatty acids with muscle and body fat show non-linear relationships, which suggests non-normal distributions of this data. This has implications for the biological interpretations of the results.
\n\u2022 The phenotypic correlation of 0.66 and the CCC of 0.54 show that the non-invasive Distell fat meter lacks accuracy and precision in this dataset.
\n\u2022 The histology results show that there were large individual differences in fat deposition in liver and health indicators of the hindgut and that most fish had scores >0.<\/p>\n![\"\"](\"http:\/\/www.medaid-h2020.eu\/wp-content\/uploads\/2020\/06\/figura-2.1a.png\")
\n\u2022 Promising large individual differences were observed for total contents of EPA (CV=0.20) and DHA (CV=0.16) on fish fed the same commercial diet. However, the proportional contents of EPA and DHA had lower CV of 0.07 and 0.06, respectively.
\n\u2022 The fatty acid data indicates that metabolic factors influence the proportional content of the omega-3 fatty acids EPA and DHA in the fillet.
\n\u2022 Muscle fat was positively correlated with the proportional contents of 18:1n-9 and 16:1n-7. These two fatty acids are products of de novo lipogenesis, but 18:1n9 also comes from the diet. This therefore indicates that fish with a higher muscle fat content has increased de novo synthesis of lipids.
\n\u2022 There is a strong positive phenotypic correlation between muscle fat and the quantitative content of the omega-3 fatty acids (as well as all other fatty acids).
\n\u2022 Muscle fat had a correlation of 0.15 with body weight, which shows that individual variation in muscle fat is independent of body weight. This is important information when setting up a selection index.
\n\u2022 The correlations between body weight and fillet weight, visceral weight, heart weight and liver weight were 0.91, 0.79, 0.47 and 0.69, respectively.
\n\u2022 The scatter plot and the phenotypic correlation of 0.59 shows that the non-invasive Distell fat meter lacks accuracy and precision to measure muscle fat contents in this dataset.
\n\u2022 There was a large individual variation in liver and hindgut histological health scores and most fish had scores >0.<\/p>\n![\"\"](\"http:\/\/www.medaid-h2020.eu\/wp-content\/uploads\/2020\/06\/figura-2.1b.png\")